CD28 Antikörper (PE)

Produktnummer ABIN118584

Dieses Anti-CD28-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von CD28 in FACS. Geeignet für Human.

Kurzübersicht für CD28 Antikörper (PE) (ABIN118584)

Target: CD28

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: YTH913-12

Produktdetails anti-CD28 Antikörper (PE)

Spezifität: This antibody recognises the human CD28 cell surface antigen, a 44 kDa glycoprotein expressed by a subset of T lymphocytes. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.

Aufreinigung: Affinity chromatography on Protein G.

Immunogen: Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with distilled water

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28

Hintergrund: Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gen-ID: 940

NCBI Accession: NP_006130

UniProt: P10747

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response